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目的探讨凋亡抑制基因Survivin和抑癌基因PTEN在原发性肝细胞癌及癌旁组织中的表达及其相关性。方法应用RT-PCR法,对50例原发性肝细胞癌和34例癌旁组织中Survivin和PTEN基因的表达情况进行定量检测。结果 Survivin在原发性肝细胞癌组织中高表达,而在癌旁组织中无表达;原发性肝细胞癌中PTEN的表达量明显低于癌旁组织;Survivin和PTEN的表达量与原发性肝细胞癌的分化程度有关,与患者发病年龄、肿瘤大小、门静脉癌栓、肝内转移无关;随着恶性程度加重,Survivin和PTEN基因表达呈显著负相关。结论 Survivin和PTEN在原发性肝细胞癌发生、发展中起着不同的作用,联合检测对原发性肝细胞癌的早期诊断、判定预后提供一定的临床依据。
Objective To investigate the expression of survivin and tumor suppressor gene PTEN in primary hepatocellular carcinoma and adjacent normal tissues. Methods The expression of Survivin and PTEN in 50 cases of primary hepatocellular carcinoma and 34 cases of paracancerous tissues were detected by RT-PCR. Results Survivin was highly expressed in primary hepatocellular carcinoma and no expression in adjacent tissues. The expression of PTEN in primary hepatocellular carcinoma was significantly lower than that in paracancerous tissues. The expression of Survivin and PTEN was correlated with the primary There was no significant correlation between the expression of Survivin and PTEN gene in hepatocellular carcinoma. The expression of Survivin and PTEN was negatively correlated with the age, tumor size, portal vein tumor thrombus and intrahepatic metastasis. Conclusions Survivin and PTEN play different roles in the occurrence and development of primary hepatocellular carcinoma. Combined detection may provide some clinical evidence for the early diagnosis and prognosis of primary hepatocellular carcinoma.