论文部分内容阅读
用３２Ｐ后标记的方法测小牛胸腺ＤＮＡ和黄曲霉素Ｂ１体外反应产物里的ＤＮＡＡＦＢ加合物，发现有４种不同的ＤＮＡ加合物，加合物含量的ＲＡＬ＝１０８（加合物）／１０７（正常核酸），对比用ＤＮＡ的４种碱基和ＡＦＢ１反应所形成的加合物，发现有３种ＤＮＡＡＦＢ１的加合物是来自鸟嘌呤碱基被修饰所形成的，占ＤＮＡ加合物总量的９０％．用定量的黄曲霉素Ｂ１腹腔注射入大白鼠中，２４ｈ取其肝、肾、肺、脾等组织，加合物的总含量分别是肝＞肾＞肺＞脾．用ＡＲＯＣＬＯＲ诱导能促进各组织中ＤＮＡ加合物的形成，但是对不同的组织有不同的促进作用．
The DNA-AFB adducts in calf thymus DNA and aflatoxin B1 in vitro reaction products were detected by the 32P labeling method. Four different DNA adducts were found. The adduct content was RAL = 108 (Adduct) / 107 (normal nucleic acid), compared with the four bases of DNA and AFB1 reaction formed adduct, found three kinds of DNA-AFB1 adducts from guanine base is modified Formed, accounting for 90% of the total amount of DNA adducts. Quantitative aflatoxin B1 was injected intraperitoneally into rats, and the liver, kidney, lung, spleen and other tissues were harvested at 24h. The total content of adducts was liver> kidney> lung> spleen. Induction with AROCLOR promotes the formation of DNA adducts in various tissues but with different promoting effects on different tissues.