论文部分内容阅读
对印度块菌Tuber indicum子实体的提取物,包括55%乙醇粗提物(ECE)、石油醚提取物(PEF)和乙酸乙酯提取物(EAF)的清除DPPH自由基和羟基自由基能力、铁离子鳌合能力以及各提取物的总酚含量等进行了研究和测定,结果显示,3种提取物的清除自由基能力和铁离子鳌合能力具有显著差异(P<0.05);ECE对DPPH自由基的清除活性最高,其EC50值为1.61g/L;EAF对羟基自由基及铁离子表现出较强的清除或螯合的能力,其EC50值分别为3.31g/L和0.70g/L;EAF的总酚含量(2.964mg GAE/g提取物)最高,其次是ECE,总酚含量为(2.618mg GAE/g提取物);PEF的清除自由基和铁离子鳌合能力较差,其总酚含量也最低(1.124mg GAE/g提取物);总酚含量与印度块菌提取物清除自由基以及鳌合铁离子的能力密切相关。
Extracts of Indian Tuber Tuber indicum fruiting body, including 55% ethanol extract (ECE), petroleum ether extract (PEF) and ethyl acetate extract (EAF), scavenged DPPH radical and hydroxyl radical ability, Ferric ion chelating ability and the total phenolic content of the extracts were studied and determined. The results showed that there was a significant difference (P <0.05) between free radical scavenging ability and iron chelation ability of the three extracts. The highest scavenging activity of free radicals was observed with an EC50 of 1.61 g / L. EAF exhibited strong ability of scavenging or chelating hydroxyl radicals and iron ions with EC50 values of 3.31 g / L and 0.70 g / L, respectively ; The total phenolic content (2.964 mg GAE / g extract) of EAF was highest, followed by ECE, the total phenolic content was (2.618 mg GAE / g extract); the free radical scavenging ability of PEF was dissociated from ferric ion, The total phenolic content was the lowest (1.124 mg GAE / g extract). The content of total phenols was closely related to the ability of Indian truffle extract to scavenge free radicals and chelate iron ions.