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目的:探讨大鼠脑缺血早期脑内3种亚型一氧化氮合酶(nitric oxide synthase,NOS)的表达及作用。方法:采用免疫组织化学方法,用三型NOS的多克隆抗体检测大鼠局灶性脑缺血15、30min及1、2、3、6 h NOS在脑内的表达情况。结果:局灶性脑缺血1 h内缺血侧基底节区内神经元型NOS(nNOS)表达上调;脑缺血1 h内在缺血区小血管壁及3 h时在缺血边缘区小血管壁出现内皮型NOS(eNOS)两次上调。局灶性脑缺血6 h内神经组织中未见诱生型NOS(iNOS)表达。结论:在脑缺血的超早期出现结构型NOS(nNOS及eNOS)的上调表达,此时可能同时存在nNOS的神经损伤作用及eNOS的神经保护作用。
AIM: To investigate the expression and effect of nitric oxide synthase (NOS) in three brain regions in early stage of cerebral ischemia in rats. Methods: Immunohistochemistry was used to detect the expression of NOS in the brain at 15, 30 min and 1, 2, 3 and 6 h after focal cerebral ischemia. Results: Within 1 h after focal cerebral ischemia, the expression of neuronal NOS (nNOS) in the ischemic basal ganglia was up-regulated. Within 1 h of cerebral ischemia, the small blood vessel wall in the ischemic area and the ischemic marginal zone at 3 h Endothelial NOS (eNOS) appears to be upregulated twice in the vessel wall. There was no induced iNOS expression in nerve tissue within 6 h after focal cerebral ischemia. CONCLUSION: The up-regulated expression of structural NOS (nNOS and eNOS) occurs in the early stage of cerebral ischemia. At this time, the neuronal damage of nNOS and the neuroprotective effect of eNOS may be present.