论文部分内容阅读
采用He-Ne激光辐照处理经增强紫外线B（UV-B）损伤的小麦幼苗,研究小麦细胞DNA中损伤产物环丁烷嘧啶二聚体（CPDs）的变化.T4核酸内切酶V能特异识别CPDs,并造成单链缺失（SSB）,SSB的含量以酶敏感位点（ESS）数表示.通过T4核酸内切酶V的酶切及琼脂糖凝胶电泳法分析测定了UV-B和He-Ne激光处理后小麦细胞DNA中ESS的含量,即CPDs的数量.结果表明,He-Ne激光的辐照能促进小麦细胞修复由UV-B辐射造成的损伤.探讨了激光作用的机制.
He-Ne laser irradiation was used to study the changes of the cyclobutane pyrimidine dimers (CPDs) in DNA of wheat cells exposed to enhanced ultraviolet B (UV-B) injury.T4 endonuclease V can be specific Identifying CPDs and resulting in single-strand deletion (SSB), the SSB content is expressed as the number of enzyme-susceptible sites (ESSs) UV-B and UV-B were determined by digestion with T4 endonuclease V and agarose gel electrophoresis The content of ESS in wheat cell DNA after He-Ne laser treatment, that is, the number of CPDs, showed that the irradiation of He-Ne laser could promote the damage of wheat cells repaired by UV-B radiation and the mechanism of laser action was discussed.