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目的 :研究纳洛酮对心肌缺血再灌注损伤的影响 ,从分子水平探讨心肌缺血再灌注损伤的保护机制。方法 :30只大白兔随机分为三组 :(1)对照组 ;(2 )心肌缺血 30分钟再灌 30分钟组 ;(3)静注纳洛酮 10分钟后缺血 30分钟再灌 30分钟组。提取心肌总RNA与经同位素标记的c foscDNA探针进行分子杂交并自显影 ,测定c fos基因mR NA水平。结果 :纳洛酮能显著抑制心肌缺血再灌注时c fos基因的表达。结论 :纳洛酮可能具有全心缺血再灌注损伤的分子保护作用。
Objective: To study the effect of naloxone on myocardial ischemia-reperfusion injury and to explore the protective mechanism of myocardial ischemia-reperfusion injury at the molecular level. Methods: Thirty rabbits were randomly divided into three groups: (1) control group; (2) myocardial ischemia for 30 minutes followed by 30 minutes of reperfusion; (3) naloxone 10 minutes after ischemia and 30 minutes of reperfusion 30 Minute group. Myocardial total RNA was extracted and labeled with isotope labeled c foscDNA probe hybridization and autoradiography to determine c fos gene mR NA levels. Results: Naloxone significantly inhibited the expression of c fos gene during myocardial ischemia-reperfusion. Conclusion: Naloxone may have the molecular protective effect of global ischemia-reperfusion injury.