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目的:通过研究氨基胍对隐睾生精细胞中p53表达的影响,探讨生精细胞凋亡的分子机制。方法:雄性SD大鼠随机分为假手术组、隐睾组、隐睾+氨基胍(Aminoguanidine,AG,iNOS抑制剂)组、隐睾+生理盐水组。后三组建立单侧隐睾模型,后两组术后分别于腹腔内注射AG和生理盐水,每天一次,定时定量。7天后处死所有大鼠,取双侧睾丸称湿重。手术侧睾丸H.E染色观察常规组织学及免疫组化检测p53的表达。结果:隐睾+AG组睾丸生精上皮较隐睾组及隐睾+生理盐水组排列有序,细胞层数厚,该组中p53表达弱于隐睾组。结论:AG可降低隐睾中p53的表达,抑制隐睾生精细胞的凋亡,提示NO可能通过增强隐睾生精细胞中p53的表达而促进生精细胞凋亡。
OBJECTIVE: To investigate the effect of aminoguanidine on the expression of p53 in cryptorchid spermatogenic cells and to explore the molecular mechanism of apoptosis of spermatogenic cells. Methods: Male Sprague-Dawley rats were randomly divided into sham operation group, cryptorchidism group, cryptorchidism + aminoguanidine (AG) and iNOS inhibitor group, cryptorchidism + saline group. The latter three groups were established unilateral cryptorchidism model, the latter two groups were injected intraperitoneally with AG and saline, once a day, regular quantitative. After 7 days, all rats were sacrificed, and bilateral testicular weight was measured. Surgical side of the testis H.E staining observed by conventional histological and immunohistochemical detection of p53 expression. Results: Cryptorchidism + AG group of testis germ cell epithelium than cryptorchid group and cryptorchid + saline group arranged in order, the number of layers of cells, p53 expression in this group weaker than cryptococcal group. Conclusion: AG can reduce the expression of p53 in cryptorchidism and inhibit the apoptosis of spermatogenic cells in cryptorchidism, suggesting that NO may promote the apoptosis of spermatogenic cells by enhancing the expression of p53 in cryptorchid spermatogenic cells.