目的　研究腺病毒介导 Ｈ Ｓ Ｖｔｋ／ Ｇ Ｃ Ｖ 系统和反义 Ｉ Ｇ Ｆ１ 联合基因治疗大鼠脑胶质瘤，并对其机制作初步分析。方法　利用腺病毒为载体观察联合 Ｈ Ｓ Ｖｔｋ／ Ｇ Ｃ Ｖ 和反义 Ｉ Ｇ Ｆ１ 离体杀伤 Ｃ６细胞的效果， 并在大鼠脑内接种 Ｃ６ 细胞第８ 天进行 Ｈ Ｓ Ｖｔｋ／ Ｇ Ｃ Ｖ 和反义 Ｉ Ｇ Ｆ１ 的原位治疗， 观察大鼠生存期变化。结果　 Ｇ Ｃ Ｖ 对转染反义 Ｉ Ｇ Ｆ１ 基因和ｔｋ 基因的 Ｃ６ 细胞较转染ｔｋ 基因的 Ｃ６ 细胞敏感。联合应用 Ｈ Ｓ Ｖｔｋ／ Ｇ Ｃ Ｖ 系统和反义 Ｉ Ｇ Ｆ１ 原位治疗脑肿瘤比单用任何一种治疗方法效果明显， 免疫组化发现联合基因治疗组肿瘤有大量 Ｃ Ｄ＋４ 、 Ｃ Ｄ＋８ 淋巴细胞浸润。结论　反义 Ｉ Ｇ Ｆ１ 基因可增强 Ｈ Ｓ Ｖｔｋ／ Ｇ Ｃ Ｖ 杀伤肿瘤的作用。 Objective To study the adenovirus-mediated H S V -tk / G C V system and antisense I G F-1 combined gene therapy of glioma in rats, and its mechanism for primary analysis. Methods The adenovirus vector was used to observe the effect of combination of H S Vtk / G C V and antisense I G F1 on vitro killing of C6 cells. C6 cells were seeded into rat brain on the 8th day for H S V tk / G C V and antisense I G F 1 in situ treatment, observed changes in the survival of rats. Results G C V was more sensitive to C6 cells transfected with antisense I G F1 gene and tk gene than C6 cells transfected with tk gene. Combination of H S V  tk / G C V system and antisense I G F  1 in situ treatment of brain tumors than any single treatment effect is obvious, immunohistochemistry found that combined gene therapy group of tumors with a large number of C D +4, C D +8 lymphocyte infiltration. Conclusion Antisense I G F-1 gene can enhance H S V  tk / G C V tumor-killing effect.