Dissecting the genetic relationships among gluten-related traits is important for high quality wheat breeding. Quantitative trait loci(QTLs) analysis for gluten strength,as measured by sedimentation volume(SV) and gluten index(GI),was performed using the QTLNetwork 2.0 software. Recombinant inbred lines(RILs) derived from the winter wheat varieties Shannong 01-35×Gaocheng 9411 were used for the study. A total of seven additive QTLs for gluten strength were identified using an unconditional analysis. QGi1 D-13 and QSv1 D-14 were detected through unconditional and conditional QTLs mapping,which explained 9.15–45.08% of the phenotypic variation. QTLs only identified under conditional QTL mapping were located in three marker intervals:WPT-3743–GLU-D1(1 D),WPT-7001–WMC258(1 B),and WPT-8682–WPT-5562(1 B). Six pairs of epistatic QTLs distributed nine chromosomes were identified. Of these,two main effect QTLs(QGi1 D-13 and QSv1 D-14) and 12 pairs of epistatic QTLs were involved in interactions with the environment. The results indicated that chromosomes 1 B and 1 D are important for the improvement of gluten strength in common wheat. The combination of conditional and unconditional QTLs mapping could be useful for a better understanding of the interdependence of different traits at the QTL molecular level. Quantitative trait loci (QTLs) analysis for gluten strength, as measured by sedimentation volume (SV) and gluten index (GI), was performed using the QTLNetwork 2.0 software . Recombinant inbred lines (RILs) derived from the winter wheat varieties Shannong 01-35 × Gaocheng 9411 were used for the study. A total of seven additive QTLs for gluten strength were identified using an unconditional analysis. QGi1 D-13 and QSv1 D- QTLs only identified under conditional QTL mapping were located in three marker intervals: WPT-3743-GLU-D1 (1 D), WPT-7001 Of these, two main effect QTLs (QGi1 D-13 and QSv1 D-14) and WPT-8682-WPT-5562 (1 B) 12 pairs of epistatic QTLs were involved in int The results indicated that chromosomes 1 B and 1 D are important for the improvement of gluten strength in common wheat. The combination of conditional and unconditional QTLs mapping could be useful for a better understanding of the interdependence of different traits at the QTL molecular level.