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目的探讨DNA倍体分析系统在良恶性支气管刷片或支气管灌洗液中的诊断价值。方法 2012年6月-2013年6月96例病理诊断明确的良恶性支气管刷片或支气管灌洗液标本,其中良性49例,恶性47例。支气管刷片由临床医生进行支气管镜检查时刷取2张刷片,支气管灌洗液标本经离心涂片,每例制成2张薄层细胞片。1张常规苏木精-伊红染色,进行常规细胞学检查;另外1张Feulgen染色,利用DNA倍体全自动图像分析仪进行DNA倍体分析。结果常规细胞学对支气管刷片或支气管灌洗液良恶性诊断的特异度、灵敏度、准确率分别为85.7%、78.7%及77.1%,而DNA倍体分析的特异度、灵敏度、准确率分别为100.0%、91.5%及95.8%,两种方法比较差异均有统计学意义(P<0.05)。结论 DNA倍体分析能够提高支气管刷片或支气管灌洗液的阳性检出率,并且在诊断良恶性病变中较常规细胞学更特异、更敏感,具有较高的价值。
Objective To investigate the diagnostic value of DNA ploidy analysis system in benign and malignant bronchial brush or bronchial lavage fluid. Methods From June 2012 to June 2013, 96 cases of benign and malignant bronchial brush or bronchial lavage fluid were identified, including 49 cases of benign and 47 cases of malignant. Bronchial brushing Bronchoscopy brushing by the clinician brush 2 brush, bronchial lavage samples by centrifugation smear, each made of two thin-sheet cells. One routine hematoxylin-eosin staining was performed for routine cytology. Another Feulgen staining was performed and DNA ploid analysis was performed using a DNA ploid automatic image analyzer. Results The specificity, sensitivity and accuracy of routine cytology in the diagnosis of benign and malignant bronchial brushing or bronchial lavage were 85.7%, 78.7% and 77.1% respectively, while the specificity, sensitivity and accuracy of DNA ploidy analysis were 100.0%, 91.5% and 95.8% respectively. There was significant difference between the two methods (P <0.05). Conclusion DNA ploidy analysis can improve the positive detection rate of bronchial brush or bronchial lavage fluid, and is more specific and sensitive than conventional cytology in the diagnosis of benign and malignant lesions, which is of high value.