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目的:观察小胶质细胞抑制剂对佐剂性关节炎大鼠脊髓促炎性细胞因子的影响。方法:蛛网膜下腔置管成功的雄性SD大鼠分别脊髓蛛网膜下腔注射(it)生理盐水(NS)和米诺环素50μg,30 min后右踝关节皮内注射完全氟氏佐剂(CFA),it,qd,连续7 d,观察CFA后0,2,6,13 d后爪热刺激回缩潜伏期(PWTL)的变化及CFA致炎后0,2,6 d给药后4 h和13 d脊髓IL-1β,IL-6,TNF-α含量变化,这些促炎性细胞因子的表达水平通过ELISA方法检测。结果:米诺环素能抑制PTWL缩短;CFA致炎后脊髓IL-1β,IL-6,TNF-α表达明显增加,米诺环素可减少相应时间点脊髓IL-1β,IL-6,TNF-α的表达水平。结论:蛛网膜下腔注射米诺环素可以减少外周炎症诱导的脊髓促炎性细胞因子的分泌;脊髓小胶质细胞可能通过促炎性细胞因子介导炎性痛觉过敏。
Objective: To observe the effect of microglial inhibitor on proinflammatory cytokines in spinal cord of rats with adjuvant arthritis. Methods: Sprague-Dawley rats were subcutaneously injected with normal saline (NS) and minocycline (50 μg) into the spinal cord of male Sprague-Dawley rats. Subcutaneous injection of complete Freunds adjuvant (CFA), it, qd for 7 consecutive days. The change of paw withdrawal latency (PWTL) at 0, 2, 6 and 13 days after CFA was observed, The levels of IL-1β, IL-6 and TNF-α in the spinal cord at day 13 and day 13 were detected by ELISA. RESULTS: Minocycline inhibited PTWL shortening. The expression of IL-1β, IL-6 and TNF-α in the spinal cord increased after CFA-induced inflammation. Minocycline decreased the expression of IL-1β, IL-6 and TNF -α expression levels. Conclusion: Subarachnoid injection of minocycline can reduce peripheral inflammation-induced secretion of proinflammatory cytokines in the spinal cord. Spinal cord microglia may mediate inflammatory hyperalgesia through pro-inflammatory cytokines.