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本文报道用紫外分光光度法直接测定药剂中的维生素C.该方法简便、快速、准确,比滴定法灵敏,不受还原性物质及制剂中通常存在的物质的干扰.方法:在波长267nm处空白作对照测定维生素C标准溶液吸光度制备校正曲线.标准溶液(0～20μg/ml)由含0～120μg/ml维生素C适当浓度的溶液1.0ml和5ml Cu(Ⅱ)-EDTA溶液在试管中混合,测定其校正曲线的斜率(S).为测定药品中维生素C,将片剂或胶囊内容物用50ml蒸馏水溶解,搅拌2～3min.混合物用蒸馏水配成100ml,过滤,先弃去几毫升滤液,再收集滤液.根据维生素C的含量和样品颜色的情况,可用蒸馏水加以稀释.对于液体样品,测量样品的体积并用蒸馏水稀释至100ml.若油质样品,测定前用蒸馏水抽提,然后再稀释至100ml.
This article reports the direct determination of vitamin C in pharmaceuticals by UV spectrophotometry.The method is simple, rapid, accurate and sensitive than titration method.It is not disturbed by the reducing substances and substances usually present in the preparation.Methods: Blank at the wavelength of 267nm The standard curve (0 ~ 20μg / ml) consisted of 1.0ml of appropriate concentration of vitamin C from 0 ~ 120μg / ml and 5ml of Cu (Ⅱ) -EDTA solution were mixed in a test tube, Determine the slope of the calibration curve (S) .For the determination of vitamin C in the drug, dissolve the contents of the tablet or capsule with 50ml of distilled water and stir for 2 ~ 3min.The mixture is made up to 100ml with distilled water, filtered, first discarded a few milliliters of filtrate, The filtrate is collected and diluted with distilled water according to the content of vitamin C and the color of the sample.For liquid samples, the volume of the sample is measured and diluted to 100 ml with distilled water.If the sample is oily, extracted with distilled water before measurement and then diluted 100ml.