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为建立大鼠肥胖、高脂血症性脂肪性肝炎 (NASH)的动物模型。取雄性SD大鼠 1 9只 ,随机分为模型组 (n =1 0 )和正常组 (n =9)。模型组喂高脂饮食 ,即普通饲料基础上加 1 0 %猪油 ,2 %胆固醇。正常组喂普通饲料。观察一般情况及血清肝功能、血脂 ,并行病理学检查。模型组体重、肝指数、血清总胆固醇、游离脂肪酸及转氨酶均显著高于正常组 ;病理学均出现弥漫性肝细胞脂肪变性 ,且 1 0 0 %存在小叶内炎症 ,80 %出现汇管区炎症 ,并有 2例出现碎屑样坏死。电镜示肝细胞索排列紊乱 ,胞浆脂滴大量出现、聚积 ,线粒体嵴肿胀 ,肝星状细胞和Kupffer细胞增生。通过 3个月的高脂肪、高胆固醇饮食成功地复制了大鼠NASH模型
To establish an animal model of obese, hyperlipidemic steatohepatitis (NASH) in rats. A total of 19 male SD rats were randomly divided into model group (n = 10) and normal group (n = 9). Model group fed high-fat diet, which is based on the general feed plus 10% lard, 2% cholesterol. Normal group fed normal feed. Observe the general situation and serum liver function, blood lipids, parallel pathological examination. The body weight, liver index, serum total cholesterol, free fatty acid and aminotransferase in the model group were significantly higher than those in the normal group. Pathological changes included diffuse hepatocellular steatosis, lobular inflammation in 100%, inflammation in portal area in 80% There were 2 cases of debris-like necrosis. Electron microscopy showed abnormal arrangement of hepatic cord cells, large amounts of lipid droplets in the cytoplasm, accumulation, swelling of mitochondria cristae, proliferation of hepatic stellate cells and Kupffer cells. Rat NASH model was successfully replicated by a 3 month high fat, high cholesterol diet