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目的 评价９９Ｔｃｍ 标记抗血栓成分（ 血小板和纤维蛋白）的单克隆抗体（ＭｃＡｂ） ＳＺ－ ５１ 和ＳＺ－ ６３，对实验性动物（ 兔、狗）股动脉和股静脉血栓模型显像的作用。方法 以亚氨基噻吩盐酸盐（２ － ＩＴ） 修饰，９９Ｔｃｍ 标记葡庚糖酸钠（ＧＨ） 配体交换法标记ＳＺ－ ５１ 和ＳＺ－ ６３。狗、兔股动脉和股静脉血栓模型制备完毕后，耳缘静脉注入９９ Ｔｃｍ － ＳＺ－ ５１或９９Ｔｃｍ － ＳＺ－ ６３ 进行ＳＰＥＣＴ 显像。９９Ｔｃｍ 标记非特异性鼠ＩｇＧ 作为阴性对照。结果 ９９Ｔｃｍ － ＳＺ－５１ 和９９Ｔｃｍ － ＳＺ－５１／６３ 对狗新鲜血栓均显示清晰，其最佳显像时间分别为注射后２ ～４ｈ 和０．５ ～２ｈ，而陈旧血栓未见显像。９９Ｔｃｍ － ＳＺ－ ６３ 兔陈旧血栓显示清晰，其最佳显像时间为注射后２ ～４ｈ。结论 ９９Ｔｃｍ － ＳＺ－ ５１ 和９９Ｔｃｍ － ＳＺ－ ６３ 具有活体内血栓定位导向能力，显像时间短，用于血栓性疾病的放免显像有一定的可行性。
Objective To evaluate the effects of monoclonal antibodies (SZ-51 and SZ-63) labeled with 99Tcm labeled anti-thrombotic agents (platelets and fibrin) on imaging of experimental animals (rabbit and dog) femoral and femoral vein thrombosis models. Methods SZ - 51 and SZ - 63 were labeled with iminothiophene hydrochloride (2 - IT) and 99Tcm labeled sodium glucoheptonate (GH). After preparation of the dog, rabbit femoral artery and femoral vein thrombosis models, 99 Tcm - SZ - 51 or 99Tcm - SZ - 63 was injected into the ear marginal vein for SPECT imaging. 99Tcm labeled non-specific murine IgG as a negative control. Results 99Tcm - SZ - 51 and 99Tcm - SZ-51/63 showed clear thrombus in fresh thrombus. The best imaging time was 2 ~ 4h and 0.5 ~ 2h after injection, while no imaging was observed in old thrombus. 99Tcm - SZ- 63 rabbit thrombosis showed a clear old, the best imaging time after injection 2 ~ 4h. Conclusion 99Tcm - SZ - 51 and 99Tcm - SZ - 63 have the ability of targeting thrombosis in vivo and the imaging time is short. It is feasible to use radiofrequency imaging in thrombotic diseases.