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银杏叶提取物中含有多种生物活性成分,对银杏叶多糖的提取纯化及结构鉴定进行了研究。对多糖产品用高效液相和气相色谱及红外进行分析,得多糖纯度为94%以上,相对分子量为12749,含有鼠李糖、阿拉伯糖、甘露糖、葡萄糖、半乳糖。其中鼠李糖:阿拉伯糖:甘露糖:葡萄糖:半乳糖=0.236:0.2104:0.1608:0.078:0.315。银杏叶多糖的特征吸收峰分别为3423cm-1(-OH),2931cm-1(C-H),1741cm-1(-COO-)酯键中(-C=O),1141~1020cm-1(-C-OH)和(-C-O-C-);834、918、763cm-1处吸收峰表明银杏叶多糖含有α-D葡萄吡喃糖苷键。
Ginkgo biloba extract contains a variety of bioactive ingredients, extraction and purification of Ginkgo biloba polysaccharide and structural identification were studied. Polysaccharide products with high performance liquid and gas chromatography and infrared analysis, the polysaccharide purity of 94% or more, the relative molecular weight of 12749, containing rhamnose, arabinose, mannose, glucose, galactose. Among them, rhamnose: arabinose: mannose: glucose: galactose = 0.236: 0.2104: 0.1608: 0.078: 0.315. The characteristic absorption peaks of Ginkgo biloba polysaccharide were 3423 cm-1 (-OH), 2931 cm-1 (CH), 1741 cm-1 (-COO-) -OH) and (-COC-); absorption peaks at 834, 918 and 763 cm-1 indicate that Ginkgo biloba polysaccharide contains α-D glucopyranosidic linkages.