传统细菌培养计数法是依据细菌表型特征来对双歧杆菌进行鉴别计数的,MTPY和BSM培养基是经常使用的双歧杆菌培养基。近年来包括点杂交、荧光原位杂交、荧光原位杂交结合流式细胞计数和荧光实时定量PCR技术在内的以核糖体及其编码基因核酸序列为鉴别基础的分子生物学技术被广泛用于双歧杆菌的定量定性分析,其中荧光原位杂交结合流式细胞计数和荧光实时定量PCR技术是目前较为理想的方法。 The traditional bacterial culture counting method is based on the bacterial phenotypic characteristics of bifidobacteria were counted, MTPY and BSM medium is commonly used Bifidobacterium medium. In recent years, molecular biology techniques including the identification of ribosomes and their coding gene nucleic acid sequences including dot blot, fluorescence in situ hybridization, fluorescence in situ hybridization combined with flow cytometry and real-time quantitative PCR techniques have been widely used in Bifidobacterium quantitative qualitative analysis, of which fluorescence in situ hybridization combined with flow cytometry and real-time quantitative PCR technology is the current more ideal method.