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目的 :检测不同破壁率的灵芝孢子的醇提效果并用色谱技术对醇提物中的抗肿瘤成分进行分析。方法 :乙醇为溶剂对灵芝孢子粉进行振荡浸提 ,醇提物依次用硅胶柱和高效液相色谱分析 ,以各部分对Hela细胞的毒性作为其抗肿瘤活性的指标。结果 :未破壁孢子 ,6 0 %～ 80 %及 99%破壁率孢子的醇提率分别为 5 % ,2 5 % ,33%。99%破壁率灵芝孢子的醇提物经硅胶柱分离 ,氯仿洗脱部分对Hela细胞无毒害作用 ;甲醇洗脱部分的抗肿瘤活性约为乙酸乙酯洗脱部分的 34倍 ;甲醇洗脱部分经高效液相色谱分析 ,得到 2种活性较强且组成较为简单的混合物(Ⅱ1和Ⅱ3 )。结论 :灵芝孢子破壁后醇提物含量远高于未破壁孢子 ;甲醇 水洗脱系统可用于灵芝孢子醇提物中抗肿瘤活性成分的RP HPLC分析。
Objective : To detect the alcohol extracting effect of Ganoderma lucidum spores with different breaking rates and to analyze the antitumor components in ethanol extracts by chromatography. Methods: Ethyl alcohol was used as the solvent to oscillate the extracts of Ganoderma lucidum spores. The alcohol extracts were analyzed by silica gel column and high-performance liquid chromatography. The toxicity of Hela cells in each part was used as an indicator of its anti-tumor activity. RESULTS: In the unbroken spores, the alcohol extraction rates of 60% to 80% and 99% of the spoilage rate were 5%, 25%, and 33%, respectively. The ethanol extract of the fungus spores with 99% disruption rate was separated on a silica gel column, and the fraction eluted with chloroform had no toxic effect on the Hela cells; the antitumor activity of the methanol-eluted fraction was approximately 34 times that of the ethyl acetate elution fraction; Some high performance liquid chromatography analysis yielded two more active and simpler mixtures (II1 and II3). Conclusion : The content of alcohol extract of ganoderma lucidum spores is much higher than that of unbroken spores. The methanol water elution system can be used for RP HPLC analysis of antitumor active components in the extracts of ganoderma lucidum spores.