用氯仿、乙醚、乙醇和盐酸等溶剂溶解一组人体胆结石 ,获取难溶物 ;用傅里叶变换红外光谱和表面增强拉曼光谱对难溶剩余物进行研究。结果显示 ,结石难溶物主要由胆红素盐和蛋白质组成 ,结石中的蛋白质的二级结构以α螺旋和无规卷曲构象为主 ,其中 ,α螺旋构象成分较多。讨论了蛋白质在胆结石形成中的作用 A series of human gallstones were dissolved in chloroform, ether, ethanol and hydrochloric acid to obtain insoluble materials. The insoluble residues were studied by Fourier transform infrared spectroscopy and surface enhanced Raman spectroscopy. The results showed that the refractory mainly composed of bilirubin salt and protein composition, the secondary structure of the protein in the stone α-helix and random coil conformation, of which, α-helix conformation more. The role of proteins in gallstone formation is discussed