论文部分内容阅读
目的:研究淫羊藿对大鼠骨量及骨髓基质干细胞增殖、分化过程中Cbfa1和Osterix表达的影响。方法:24只雌性SD大鼠被随机分成观察组和对照组各12只,其中观察组给予淫羊藿0.5 g/0.1 kg,每天1次灌胃,持续两周;对照组给予相同剂量的生理盐水。于分组时间将大鼠处死,取右侧股骨远侧段用于骨组织形态计量学检测;抽取左侧股骨骨髓用于细胞培养,于培养21 d时提取细胞总RNA,经PCR方法分离获得cbfa1,osterix扩增产物。结果两组大鼠的骨形态计量学进行比较,观察结果表明,观察组大鼠骨组织计量学动态参数以及静态参数BV/TV、BFR/BS、tb.th、MAR比着对照组明显要高,差异具有统计学意义(P<0.05)。两组大鼠的Cbfa1,Cbfb和OSX m RNA表达的差异比较,研究组Cb fa1 m RNA,OSX m RNA在各时间点的表达均明显增加(P<0.05),并且这种差异随SIM作用时间增加而增强,研究组与对照组Cbfbm RNA差异无统计学意义(P>0.05)两组大鼠的骨形态蛋白-2以及转化因子β1相比,骨形态蛋白-2观察组明显高于对照组,差异存在统计学意义(P<0.05),转化因子β1比较观察组明显高于对照组,差异存在统计学意义(P<0.05)。结论:骨组织形态计量学方法显示淫羊藿组促进骨量增加;细胞分子生物学显示淫羊藿能够促进成骨细胞分化增殖作用,为中药抗骨质疏松提供组织细胞和分子生物学基础证据。
OBJECTIVE: To study the effect of Epimedium on the bone mass and the expression of Cbfa1 and Osterix during the proliferation and differentiation of bone marrow stromal cells in rats. Methods: Twenty-four female Sprague-Dawley rats were randomly divided into observation group and control group with 12 rats in each group. The observation group was treated with Epimedium 0.5 g / 0.1 kg once daily for 2 weeks. The control group received the same dose of physiological brine. The rats were sacrificed at the time of grouping, and the distal femur of the right femur was taken for bone histomorphometry. Left femur bone marrow was collected for cell culture, total cellular RNA was extracted on day 21 and cbfa1 , osterix amplification product. Results The bone histomorphometry of two groups of rats were compared. The results showed that the dynamic parameters of bones and the static parameters BV / TV, BFR / BS, tb.th and MAR in the observation group were significantly higher than those in the control group , The difference was statistically significant (P <0.05). Compared with the expression of Cbfa1, Cbfb and OSX m RNA in two groups, the expressions of Cb fa1 m RNA and OSX m RNA in the study group were significantly increased at all time points (P <0.05), and the differences were associated with the duration of SIM effect (P> 0.05) Compared with control group, the expression of Cbfbm RNA in study group and control group was not significantly different (P> 0.05). Compared with Bone Morphogenetic Protein-2 , The difference was statistically significant (P <0.05), the conversion factor β1 was significantly higher than the control group, the difference was statistically significant (P <0.05). Conclusion: Bone histomorphometric analysis showed that epimedium promoted the increase of bone mass. The molecular biology of Epimedium showed that epimedium promoted the differentiation and proliferation of osteoblasts, which provided the basic evidence for the anti-osteoporosis of Chinese traditional medicine for cell and molecular biology .