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观察小檗胺 ( Ber)对高钾除极 ,Bay K8644,5-羟色胺 ( 5- HT)及咖啡因升高细胞内钙水平( [Ca2 + ]i)的影响。以 Fluo- 3/AM负载家兔培养的主动脉平滑肌细胞 ( VSMC) ,共聚焦显微术测定[Ca2 + ]i,结果以荧光强度 ( FI)表示 .结果 :( 1 )在细胞外钙为 1 .3mmol· L-1时 ,VSMC胞浆静息 FI明显高于核区 ,且不受 Ber的影响 . ( 2 ) Ber 1 0 -1 0 0 μmol·L-1预处理可抑制 KCl60 mmol·L-1或Bay K86441 0 0 μmol·L-1升高的 [Ca2 + ]i,抑制 5-HT 1μmol· L-1升高 [Ca2 + ]i 的持续相 ,但不影响[Ca2 + ]i 的一过性升高。维拉帕米 1 0 μmol· L-1具有相似作用 . ( 3)在无钙 Hanks液中 ,Ber预处理对咖啡因 1 0 0 mmol·L-1升高的 [Ca2 + ]i 无明显抑制作用。结果表明 ,Ber可阻断外钙内流 ,但不抑制内钙释放 ,这可能与 Ber阻断电压依赖性钙通道和受体依赖性钙通道的作用有关 .
To observe the effect of Ber on the intracellular calcium level ([Ca2 +] i) by high potassium depolarization, Bay K8644 serotonin (5- HT) and caffeine. The aorta smooth muscle cells (VSMCs) were cultured with Fluo-3 / AM and the [Ca2 +] i was measured by confocal microscopy.The results were expressed as fluorescence intensity.RESULTS: (1) 1.3 mmol · L-1, the resting FI of cytoplasm of VSMC was significantly higher than that of the nucleus, and was not affected by Ber. (2) Ber 1 0 -1 0 0 μmol·L -1 pretreatment inhibited KCl 60 mmol · L-1 or Bay K86441 increased [Ca2 +] i at 0 μmol·L-1 and inhibited the increase of [Ca2 +] i in 5-HT 1 μmol · L-1 but did not affect [Ca2 +] i The transient increase. Verapamil 10 μmol · L-1 had a similar effect. (3) In the calcium-free Hanks solution, Ber pretreatment did not significantly inhibit the increase of [Ca 2+] i of caffeine in 100 mmol·L -1 effect. The results show that Ber can block the influx of extracellular calcium, but does not inhibit the release of endogenous calcium, which may be related to the role of Ber in blocking voltage-dependent calcium channels and receptor-dependent calcium channels.