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[目的 ]探讨补充加氯控制旅客列车二次供水微生物污染的效果。 [方法 ]对实验室条件下用脱氯自来水制成的人工污染大肠埃希氏菌水样及现场旅客列车水箱水进行二次补充加氯 ,分别用 3,3′,5 ,5′-四甲基联苯胺分光光度法和半乳糖苷酶膜荧光分析法检测水中游离性余氯和总大肠菌群 (TC)。 [结果 ]实验室条件下脱氯自来水加氯后水中游离性余氯≥ 0 .0 9mg/ L ,5 min可全部杀灭人工污染的大肠杆菌 ;列车水箱水经三种方式补充加氯 (一次性加氯 2 .4mg/ L ,多次加氯 0 .6 mg/ L ,间歇加氯 1.2 mg/ L )水中游离余氯从 0 .0 2～ 0 .0 4mg/ L上升至 0 .5 6～ 0 .73mg/ L,首次加氯 30 m in～ 11h后水中 TC仍未检出。 [结论 ]旅客列车二次供水系统补充加氯可提高水中游离性余氯水平 ,防止粪源性微生物的再次污染。
[Objective] The aim was to discuss the effect of adding chlorination to control the secondary pollution of passenger water supply. [Method] The secondarily added chlorination of artificially contaminated Escherichia coli water samples and passenger water tank of passenger train made from dechlorinated tap water under laboratory conditions were respectively treated with 3,3 ’, 5,5’-tetra Methylbenzidine spectrophotometry and galactosidase membrane fluorescence analysis of free residual chlorine and total coliform (TC) in water. [Result] The free residual chlorine in the water after dechlorination tap water chlorination under laboratory condition was ≥ 0 9mg / L for 5 min, which could completely kill artificial contaminated E. coli. The train water tank was replenished with three kinds of chlorination Free chlorine in water increased from 0 .0 2 ~ 0. 0 4mg / L to 0. 56 ~ 0 .73mg / L, the first time chlorination 30min ~ 11h TC in water has not yet been detected. [Conclusion] Replenishment of chlorine in secondary water supply system of passenger train can increase free chlorine level in water and prevent re-contamination of fecal-origin microorganisms.