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目的:探讨不同剂量梅术溃结灌肠汤对溃疡性结肠炎大鼠肝、肾的影响,寻求安全、有效的治疗剂量。方法:体重(200±20)g的雄性、健康的大鼠50只随机分为:空白组、模型组、药物低、中、高剂量组各10只;空白组给予基础饲料,其余4组给予高脂高糖饲料,治疗组每日以低、中、高不同剂量药物灌胃;空白、模型组同时用与各药物治疗组相同用量的生理盐水灌胃,每日1次;各组均自由饮水,干预6周,处死动物,观察各组动物肝、肾病理改变。结果:1)模型组、低剂量组肝肾均显示正常;2)高剂量组肾脏基本正常,中剂量组肾盂区轻度变性;3)高剂量组肝细胞萎缩伴自溶及轻度瘀血,中剂量组肝细胞轻度萎缩。结论:梅术溃结灌肠汤在人体推荐剂量的1.56倍范围内使用对肝肾是没有危害的;而在超过人体推荐剂量的3.13倍、6.25倍下灌胃使用则会对肝肾产生损伤,尤其是对肝脏的损伤更为明显。
Objective: To investigate the effect of different doses of Mei Shu Rou Jie enema soup on liver and kidney in rats with ulcerative colitis, and to seek a safe and effective therapeutic dose. Methods: Fifty male and healthy rats with body weight (200 ± 20) g were randomly divided into 10 groups: blank group, model group and low, medium and high dose groups. The blank group was given basic diet and the other 4 groups were given High-fat and high-sugar diet, the treatment group with low, medium and high doses of different daily doses of gavage; blank, while the model group with the same amount of each drug treatment group with normal saline gavage once daily; Drinking water, intervention for 6 weeks, animals were sacrificed, liver and kidney pathological changes in each group were observed. Results: 1) The model group and the low-dose group showed normal liver and kidney; 2) The high-dose kidney group was normal and the renal pelvis area of the middle dose group was mildly degenerated; 3) The high-dose group of hepatocyte atrophy with autolysis and mild blood stasis , Moderate dose of hepatocytes mild atrophy. Conclusion: The curative effect of Mei Shu Jie Jie Enema Decoction is 1.56 times of the recommended dose of human body, which is harmless to the liver and kidney. However, if it is used more than 3.13 times and 6.25 times of the recommended dose of human body, it will damage the liver and kidney, Especially for the liver damage more obvious.