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【目的】研究益气除痰方对肺癌A549细胞凋亡的影响及其分子作用机制。【方法】应用四甲基偶氮唑盐(MTT)比色法检测益气除痰方对A549细胞的生长抑制作用,流式细胞术碘化丙啶(PI)单染法测定细胞凋亡率,逆转录—聚合酶链反应(RT-PCR)法测定用药前后调亡相关基因p53、bcl-2、bax mRNA表达的变化。【结果】益气除痰方含药血清对A549细胞具有生长抑制作用,且具有一定的时间—浓度依赖性;流式细胞术结果显示:益气除痰方含药血清对A549细胞具有显著诱导凋亡的作用,其低、中、高剂量组凋亡率分别为(8.40±1.67)%、(15.80±2.95)%、(27.4±0.96)%(P<0.05);RT-PCR结果显示:益气除痰方可显著上调p53 mRNA、bax mRNA表达,下调bcl-2 mRNA表达(P<0.05或P<0.01)。【结论】益气除痰方可促使A549细胞凋亡,其机制可能与提高p53表达,下调bcl-2/bax比例有关。
【Objective】 To investigate the effect of Yiqi Chu Tan Recipe on the apoptosis of lung cancer A549 cells and its molecular mechanism. 【Method】 MTT assay was used to detect the growth inhibition of Yiqi Chu Tan Prescription on A549 cells. Flow cytometry was used to determine the apoptosis rate by propidium iodide (PI) staining The expressions of p53, bcl-2 and bax mRNA were detected by reverse transcription-polymerase chain reaction (RT-PCR) before and after treatment. 【Results】 The serum containing Yiqi Chu Tan Prescription could inhibit the growth of A549 cells in a time-and concentration-dependent manner. The results of flow cytometry showed that the serum containing Yiqi Chu Tan Decoction significantly induced A549 cells The apoptotic rates in the low, medium and high dose groups were (8.40 ± 1.67)%, (15.80 ± 2.95)% and (27.4 ± 0.96)%, respectively (P <0.05) Yiqi Zhitan can significantly up-regulate the expression of p53 mRNA and bax mRNA, and down-regulate the expression of bcl-2 mRNA (P <0.05 or P <0.01). 【Conclusion】 Yiqi Chu Tan can promote the apoptosis of A549 cells. The mechanism may be related to the increase of p53 expression and the decrease of bcl-2 / bax ratio.