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目的探讨吸入印刷油墨挥发物对小鼠肝肾功能的损伤。方法 30只昆明种雄性小鼠,随机分为对照组、新闻纸油墨组和铜版纸油墨组,每组10只。采用静式染毒方法,新闻纸油墨组平均挥发浓度为152mg/m3,其中苯系物浓度为69.9mg/m3(含苯2.7mg/m3),烯烃浓度为15.7mg/m3,烷烃浓度为68.5mg/m3;铜版纸油墨平均挥发浓度为270mg/m3,其中苯系物浓度为106.9mg/m3(含苯4.05mg/m3),烯烃浓度为38.8mg/m3,烷烃浓度为124.5mg/m3;对照组不加任何物质。吸入染毒2h,1次/d,每周染毒6d,染毒30d后处死小鼠。检测小鼠血清中天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、肌酐(Cr)、尿素氮(BUN)、超氧化物歧化酶(SOD)和丙二醛(MDA)的含量,并对小鼠进行肝肾病理分析。结果新闻纸油墨组和铜版纸油墨组小鼠肝组织中ALT及MDA含量与对照组比较均升高,SOD活力均降低,差异有统计学意义(P<0.05)。新闻纸油墨组和铜版纸油墨组肾组织中BUN含量与SOD活力高于对照组,差异均有统计学意义(P<0.05)。新闻纸油墨组肝病理可见,肝组织有轻度空泡变性,肝细胞肿胀,胞浆轻度水肿;肾病理可见肾近曲小管轻度水肿,胞质疏松,肾小球尚正常。铜版纸油墨组可见肝细胞索排列轻度紊乱,中央静脉为中心出现轻度弥漫性病理改变,肝细胞有中度空泡变性,肝细胞轻度肿胀,胞质水肿;肾组织可见肾小球轻度萎缩,肾小管上皮细胞水肿,胞浆疏松。结论反复吸入油墨挥发物对小鼠的肝肾功能有损伤,但无严重的器质性病变。
Objective To investigate the damage of hepatic and renal function in mice by inhalation of printing ink volatiles. Methods Thirty male Kunming mice were randomly divided into control group, newsprint ink group and coated paper ink group, with 10 mice in each group. The average volatility of newsprint ink group was 152mg / m3, including 69.9mg / m3 of benzene, 2.7mg / m3 of benzene, 15.7mg / m3 of alkene and 68.5mg of alkane / m3; the average volatilization concentration of coated paper ink is 270mg / m3, of which the concentration of benzene series is 106.9mg / m3 (benzene 4.05mg / m3), the concentration of olefin is 38.8mg / m3 and the concentration of alkane is 124.5mg / m3; Group without any substance. Inhalation exposure 2h, 1 / d, weekly exposure 6d, mice were sacrificed 30d after exposure. The contents of aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatinine (Cr), blood urea nitrogen (BUN), superoxide dismutase (SOD) and malondialdehyde (MDA) , And liver and kidney pathological analysis of mice. Results Compared with the control group, the contents of ALT and MDA in the liver tissue of the newsprint ink group and the coated paper ink group were all increased, and the SOD activity was decreased. The difference was statistically significant (P <0.05). The content of BUN and the activity of SOD in the tissue of the newsprint ink group and the coated paper ink group were higher than those of the control group, the difference was statistically significant (P <0.05). Newsprint ink group liver pathology visible liver tissue mild vacuolar degeneration, hepatocyte swelling, cytoplasmic mild edema; renal pathology shows mild renal proximal tubule edema, loose cytoplasm, glomerular is still normal. The coated paper ink group showed slight disorder of the arrangement of the liver cell cable, mild diffuse pathological changes appeared in the center of the central vein, mild vacuolar degeneration of hepatocytes, mild swelling of hepatocytes and cytoplasmic edema; Mild atrophy, tubular epithelial cell edema, cytoplasm loose. Conclusion Repeated inhalation of ink volatiles on mice liver and kidney function damage, but no serious organic disease.