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为深入探究欧美杂交杨(Populus nigra×P.deltoides)感病的分子机理,以及抗病基因在发病过程中所起的作用,克隆了欧美杂交杨LRR3蛋白编码基因Pnd-LRR3。对Pnd-LRR3基因进行了生物信息学分析,并对其在抗锈菌过程中的功能进行了研究。Q-PCR结果显示,强致病性的落叶松-杨栅锈菌(Melampsora larici-populina)E4菌株接种6 h,Pnd-LRR3基因表达量上调增幅较大,168 h为显著下调。说明Pnd-LRR3基因在E4侵染过程中起到一定抗性作用,但最终无法阻止锈菌的侵染。
In order to further explore the molecular mechanism of susceptibility to Populus nigra × P. deltoides and the role of disease resistance genes in the pathogenesis, the Pnd-LRR3 gene was cloned from European and American poplar hybrids. Bioinformatics analysis of Pnd-LRR3 gene was carried out and the function of Pnd-LRR3 in rust resistance was studied. The result of Q-PCR showed that the expression of Pnd-LRR3 gene increased significantly after 168 h of inoculation for 6 h, and the strong pathogenicity of larvae of E. larici-populina was significantly down-regulated at 168 h. Pnd-LRR3 gene in the E4 infection play a certain resistance, but ultimately can not stop the rust infection.