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A new technology has been established to screen CTL epitope by using molecular simulation and mo-lecular dynamics. By using this technology, four peptides of MAGE-2 were screened and further identified by peptide binding assay and cytotoxic assay. Results showed that three candidate peptides had higher affinity to HLA-A2 molecule and were able to pulse CTL activities in vitro in human PBMC. The results indicated that identification of CTL epi-topes with computer assistant could overcome the compli-cated experimental steps, particularly the peptide elution step, and could directly observe peptide-MHC-Ⅰ compound binding pattern and could be used to screen CTL epitopes quickly.
By using this technology, four peptides of MAGE-2 were screened and further identified by peptide binding assay and cytotoxic assay. Results showed that three candidate peptides had higher affinity to HLA-A2 molecule and were able to pulse CTL activities in vitro in human PBMC. The results indicated that identification of CTL epi-topes with computer assistant could overcome the compli-cated experimental steps, particularly the peptide elution step, and could directly directly observed peptide-MHC-I compound binding pattern and could be used to screen CTL epitopes quickly.