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目的评价二甲基亚砜(DMSO)、地塞米松对于乙型肝炎病毒(HBV)感染HepCHLine-4细胞能力的影响。方法 HBV感染前将HepCHLine-4细胞分为DMSO处理组(A组)、地塞米松处理组(B组)、DMSO及地塞米松处理组(C组)和对照组(D组)。含胎牛血清100mL/L的细胞培养基中,A组添加20mL/L DMSO,B组添加5×10-5mol/L地塞米松,C组添加20mL/L DMSO及5×10-5mol/L地塞米松,D组不添加上述两种试剂。用相应培养基培养各组细胞4d以备病毒感染。将HBV病毒颗粒加入各组细胞中于37℃中孵育24h。电化学发光法检测感染后各组细胞培养上清中HBsAg和HBeAg的滴度;荧光定量PCR检测感染后各组细胞培养上清的HBV DNA。结果 DMSO处理组HBsAg和HBeAg的滴度相对较高;地塞米松处理组HBV DNA值相对较高;DMSO及地塞米松处理组HBsAg和HBeAg的滴度及HBV DNA值均较高,其最高值分别是125.790IU/mL,4.784S/Co,5.930×105cop-ies/mL;对照组HBsAg和HBeAg的滴度及HBV DNA值均较低,其最高值分别是85.490IU/mL,1.896S/Co,3.729×104copies/mL。结论 DMSO、地塞米松有提高HepCHLine-4细胞被HBV自然感染能力的趋势。
Objective To evaluate the effect of dimethyl sulfoxide (DMSO) and dexamethasone on the ability of HepCHLine-4 cells to infect Hepatitis B virus (HBV). Methods HepCHLine-4 cells were divided into DMSO group (group A), dexamethasone group (group B), DMSO group and dexamethasone group (group C) and control group (group D) before HBV infection. In the cell culture medium containing 100mL / L fetal bovine serum, 20mL / L DMSO was added in group A, 5 × 10-5mol / L dexamethasone in group B, 20mL / L DMSO and 5 × 10-5mol / L Dexamethasone, D group did not add the above two reagents. Each group of cells was cultured for 4 days with the corresponding culture medium for virus infection. HBV virus particles were added to each group of cells and incubated at 37 ° C for 24 hours. The HBsAg and HBeAg titers in the cell culture supernatants of all groups were detected by electrochemiluminescence. HBV DNA in the cell culture supernatants of all groups were detected by fluorescence quantitative PCR. Results The titer of HBsAg and HBeAg in DMSO treated group was relatively high, while that in dexamethasone treated group was relatively high. The titer and HBV DNA value of HBsAg and HBeAg in DMSO and dexamethasone treated groups were higher, Respectively. The titer of HBsAg and HBeAg and HBV DNA in the control group were all lower, with the highest values of 85.490IU / mL and 1.896S / Co , 3.729 × 104copies / mL. Conclusion DMSO and dexamethasone have the potential to improve the ability of HepCHLine-4 cells to naturally infect HBV.