目的探讨睾丸精原细胞瘤临床病理特点及免疫表型,进而判断其生物学行为及患者预后情况。方法应用HE及免疫组织化学SP法对(2012年10月-2016年5月)安徽省立医院已确诊的43例精原细胞瘤的病理形态进行分析总结。结果经查阅,确诊43例精原细胞瘤包括3种类型,经典型精原细胞瘤37例、精母细胞型精原细胞瘤4例、间变型精原细胞瘤2例。(1)经典型精原细胞瘤肿瘤细胞主要呈片状、小巢状、条索状、微囊状生长,间质纤维血管分割,淋巴样细胞浸润,可见不同程度的肉芽肿性反应;PLAP+,CD117+,CK-,D2-40+,CD30-,AFP-,HCG-,CD34-,抑制素-;(2)精母细胞型精原细胞瘤无明显的肉芽肿性反应,淋巴细胞浸润罕见;PLAP-,CD117少数+,CK-,D2-40-,CD30-,AFP-,HCG-,CD34-,抑制素-;(3)间变型精原细胞瘤可见出血、坏死,瘤细胞异型明显,核分裂像多见。PLAP+,CD117少数+,CK-,D2-40+,CD30-,AFP-,HCG-,CD34-,抑制素-。结论不同病理类型的精原细胞瘤的组织学结构和免疫表型各具有特征性,正确的分型对于临床治疗及判断预后有一定的指导作用。 Objective To investigate the clinicopathological features and immunophenotype of testicular seminoma and to determine its biological behavior and prognosis. Methods The pathological changes of 43 cases of seminoma diagnosed in Anhui Provincial Hospital from October 2012 to May 2016 were analyzed by HE and immunohistochemistry SP method. Results After examination, 43 cases of seminoma were identified including 3 types, 37 cases of classic seminoma, 4 cases of spermatogonia and 2 cases of anaplastic seminoma. (1) The classic seminoma tumor cells showed flake, small nests, cords, microcapsules, interstitial fibrosis and infiltration of lymphoid cells, showing granulomatous reaction of varying degrees; PLAP + , CD117 +, CK-, D2-40 +, CD30-, AFP-, HCG-, CD34-, inhibin-; (2) There was no obvious granulomatous reaction in spermatogonia, ; PLAP-, CD117 minority +, CK-, D2-40-, CD30-, AFP-, HCG-, CD34-, inhibin-; (3) Metamorphic seminoma showed bleeding, necrosis, tumor cell atypia , Mitotic image more common. PLAP +, CD117 Minority +, CK-, D2-40 +, CD30-, AFP-, HCG-, CD34-, Inhibin-. Conclusion The histological structures and immunophenotypes of different pathological types of seminoma have their own characteristics. The correct classification can guide clinical treatment and prognosis.