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目的 探讨缺氧耐受形成机制。方法 高压液相法测定缺氧耐受形成中小鼠各脑区 L-天门冬氨酸(L - ASP)、L -谷氨酸 (L - GL U )、甘氨酸 (GL Y)、γ-氨基丁酸 (γ- GABA)含量变化。结果 L - ASP、L - GL U含量 :在端脑 ,1次缺氧组与正常对照组比二者均有升高趋势 ,3、4次缺氧组与 1次缺氧组比有下降趋势。在脑干、海马 ,各组之间无统计学差异。在小脑 ,3、4次缺氧组二者与正常对照组比均有明显降低。在间脑 ,随着缺氧次数的增加 L-ASP逐渐下降 ,4次缺氧组与 1次缺氧组及正常对照组比明显降低。各脑区 L - GL Y含量均随着缺氧次数的增多而增高 ,端脑、间脑的含量在缺氧 4次组有明显回降。各脑区 γ- GABA含量在缺氧耐受形成中均未见明显变化。结论 γ- GABA与缺氧耐受形成无明显关系 ;部分脑区 L - ASP、L - GL U的减少及 GL Y的回降促进了缺血耐受的形成。
Objective To investigate the mechanism of hypoxia tolerance. Methods The levels of L - aspartate (L - ASP), L - glutamic acid (GLU), glycine (GL Y), γ - aminobutyric acid Changes in acid (γ-GABA) content. Results The content of L - ASP and L - GL U in both the hypoxic group and the primary hypoxic group tended to increase. . In the brain stem, hippocampus, no significant difference between the groups. In cerebellum, 3,4 and hypoxic group both compared with the normal control group were significantly lower. In the diencephalon, L-ASP gradually decreased with the increase of hypoxia frequency, and the ratio of hypoxia group to hypoxia group and normal control group decreased significantly. The content of L - GL Y in each brain region increased with the increase of hypoxia frequency, while the content of telencephalon and diencephalon decreased significantly in hypoxic group 4. Γ-GABA content in all brain regions in the formation of hypoxia did not change significantly. Conclusion γ - GABA has no obvious relationship with hypoxia tolerance. The decrease of L - ASP and L - GL U in part of cerebral areas and the fall of GL Y promote the formation of ischemic tolerance.