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以4个大白菜品种和4个结球甘蓝品种为试材,对位于芸薹属结球甘蓝C基因组9个连锁群的458个InDel标记进行PCR筛选,筛选出286个结球甘蓝相对于大白菜的特异InDel标记,其中位于C01连锁群31个,C02连锁群45个,C03连锁群54个,C04连锁群24个,C05连锁群22个,C06连锁群20个,C07连锁群32个,C08连锁群34个,C09连锁群24个。利用结球甘蓝C02连锁群特异InDel标记对实验室创建的大白菜—结球甘蓝易位系自交后代进行了初步鉴定,明确了外源甘蓝染色体片段的物理位置在18 096 729~18 786 494 bp之间的689 764 bp区域内。
Using 4 Chinese cabbage varieties and 4 cabbage varieties as test materials, 458 InDel markers located in 9 linkage groups of Brassica oleracea C genome were screened by PCR, and 286 cabbage varieties were screened out, The results showed that there were 31 C01 linkage groups, 45 C02 linkage groups, 54 C03 linkage groups, 24 C04 linkage groups, 22 C05 linkage groups, 20 C06 linkage groups and 32 C07 linkage groups. 34 C08 linkage groups and 24 C09 linkage groups. Based on the InDel marker of C02 linkage group in cabbage, the self-progeny of Chinese cabbage-Brassica campestris translocation line was initially identified. The physical location of the exogenous cabbage chromosome fragment was determined from 18 096 729 to 18 786 494 bp within the 689 764 bp region.