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目的:探讨Toll样受体2(toll-like receptor 2,TLR2)和Toll样受体4(toll-like receptor 4,TLR4)在人牙龈上皮细胞(human gingival epithelial cells,HGECs)的表达情况,以及上述受体在脂多糖(lipopolysaccharides,LPS)刺激下表达水平的改变。方法:采用逆转录PCR技术检测TLR2、TLR4在HGECs的基因表达,采用real-time PCR技术和流式细胞技术检测1μg/ml牙龈卟啉单胞菌(Porphyromonas gingivalis,P.gingivalis)、LPS或1μg/ml大肠杆菌(Escherichia coli,E.coli)LPS刺激后,该细胞TLR2、TLR4表达水平的改变。结果:来自不同个体的HGECs均表达TLR2,TLR4 mRNA。1μg/ml P.gingivalis LPS刺激后,TLR2基因和蛋白表达水平均明显增高(P<0.05);1μg/ml E.coli LPS刺激后,TLR4基因和蛋白表达水平明显增高(P<0.05)。结论:TLR2、TLR4在HGECs存在稳定表达,并能被LPS的刺激活化,提示上述两种受体可能参与了牙周组织的炎症和免疫反应。
Objective: To investigate the expression of toll-like receptor 2 (TLR2) and toll-like receptor 4 (TLR4) in human gingival epithelial cells (HGECs) The above receptors were changed under the stimulation of lipopolysaccharides (LPS). Methods: The gene expression of TLR2 and TLR4 in HGECs was detected by reverse transcription polymerase chain reaction (RT-PCR). Real-time PCR and flow cytometry were used to detect 1μg / ml Porphyromonas gingivalis (P.gingivalis), LPS or 1μg / ml Escherichia coli (Escherichia coli, LPS stimulation, the cells TLR2, TLR4 expression levels change. Results: HGECs from different individuals all expressed TLR2 and TLR4 mRNA. The TLR2 gene and protein expression levels were significantly increased after stimulation with 1 μg / ml P. gingivalis LPS (P <0.05). After stimulation with 1 μg / ml E. coli LPS, the expression of TLR4 gene and protein were significantly increased (P <0.05). CONCLUSION: TLR2 and TLR4 are stably expressed in HGECs and can be activated by LPS stimulation, suggesting that these two receptors may be involved in the inflammation and immune responses of periodontal tissues.