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广西地处祖国南疆,位于北纬20°54′,至26°10′之间,气候温和,雨量充沛,大豆锈病周年发生;但时轻时重仍普遍存在.为了探明引起病害轻重的原因和今后开展测报,我们特趁南方冬春自然温度偏低的有利时机,利用温箱调节,先对温度与发病的关系进行了一些观察.观察分三步进行.最初,拉开温距,设15、20、25、30℃;及后,再缩小温距,在25℃前后分别设18、20、22、24℃和25、26、27、28℃两段观察.各处理温度变幅±1℃,每处理复叶2片,又重复1次.供试大豆品种桂豆8901.观察方法采用谈宇俊、孙永亮(1989)的离体复叶人工接种保湿法.保温皿直径15cm,接种液在10×10t倍显微镜下每视野含45～50个夏孢于,喷雾接种工具采用
Guangxi is located in the southern border of the motherland, located between latitudes 20 ° 54 ’and 26 ° 10’ north latitude, with a mild climate and abundant rainfall. The rust of soybean occurs annually but when the light is heavy it still prevails. And in the future to carry out the report, we particularly take advantage of the favorable winter and spring natural temperature in the south, the use of thermostat adjustment, the relationship between temperature and the incidence of some observations.Observation in three steps.First, opened the temperature, set 15, 20, 25 and 30 ° C, and then the temperature was reduced again, and the temperature was set at 18, 20, 22, 24 and 25, 26, 27 and 28 ℃ before and after 25 ℃, 1 ℃, each treatment of compound leaf 2, repeat 1. The test soybean varieties Gui Dou 8901. Observation method Talking Yu Jun, Sun Yongliang (1989) in vitro compound leaf artificial inoculation moisturizing method. Insulation dish diameter 15cm, Inoculum in 10 × 10t times under the microscope each field containing 45 to 50 summer spore in spray vaccination tools