论文部分内容阅读
目的:确定黄芪有效部位的复合型纯化工艺。方法:采用正交试验设计法,以多糖收率、多糖含量为指标,优选了多糖醇沉和除蛋白工艺;以单因素考察法优选了黄芪总皂苷的纯化工艺。结果:总多糖最佳醇沉工艺为醇沉前生药量与浓缩液体积比1∶1,醇沉温度40℃,加入3倍量95%的工业乙醇,醇沉静置18 h,粗多糖除蛋白的最佳工艺为配制质量浓度为15 g.L-1的粗多糖溶液,加入粗多糖溶液1/3体积的Sevage试剂,连续脱除2次;大孔树脂纯化黄芪总皂苷的最佳工艺为使用D101型树脂,树脂-药材比为5∶2,以4BV水洗除杂,再以4 BV 70%乙醇洗脱,收集乙醇洗脱液,蒸干即可。结论:此法优选出的工艺可以实现黄芪总多糖、总皂苷的次序提取。工艺流程科学、经济,可有效节约资源,指导工业生产。
Objective: To determine the complex purification process of the active fractions of Astragalus. Methods: Orthogonal design method was used to polysaccharide yield, polysaccharide content as an indicator, the polysaccharide alcohol precipitation and protein removal process was optimized. The purification process of total astragalus saponins was optimized by single factor assay. Results: The optimal alcohol precipitation process of total polysaccharides was as follows: the volume ratio of crude drug to concentrate before alcohol precipitation was 1: 1, alcohol precipitation temperature was 40 ℃, 3 times of 95% industrial ethanol was added, The optimum conditions for the preparation of the concentration of 15 gL-1 crude polysaccharide solution, adding 1/3 volume of the crude polysaccharide solution Sevage reagent, continuous removal of 2 times; macroporous resin purification of Astragalus total saponins using D101 Type resin, resin - medicine ratio of 5: 2, with 4BV water wash impurity, and then 4 BV 70% ethanol, ethanol eluent was collected, evaporated to dry. Conclusion: The process of this method can be optimized to achieve the total extraction of Astragalus polysaccharides, total saponins. Scientific and economical process can effectively save resources and guide industrial production.