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为获大量摩西球囊霉(Glomous mosseae,简称G.m)的繁殖体,以高丹草(Sudangrass hyrid L.)为宿主植株接种G.m,盆栽培养12周,研究6种基质处理对G.m的繁殖参数和高丹草的生长参数的影响,以及基质理化性质、脲酶、蔗糖酶和酸性磷酸酶的活性变化。结果发现,基质Ⅵ处理后G.m的繁殖参数和生长参数均高于其他处理,其碱解氮、速效磷、速效钾、pH和有机质含量分别为121.5 mg/kg,13.47 mg/kg,106.8 mg/kg,6.9和32.3%。相关性分析显示,G.m的定殖率与菌根侵染率和孢子密度呈正相关,与接种前基质的碱解氮、速效钾和有机质含量呈负相关,与速效磷和pH呈正相关;与收获后基质的碱解氮、速效磷、速效钾、有机质、脲酶、蔗糖酶和酸性磷酸酶活性呈负相关,与pH呈正相关。侵染率和孢子密度与接种前基质理化指标呈正相关,与速效磷的正相关系数最高,其次是碱解氮;侵染率和孢子密度与收获后基质的碱解氮、速效钾、pH、有机质和酸性磷酸酶活性呈正相关,与脲酶和蔗糖酶活性呈负相关。G.m的优良繁殖基质为草炭土:田间土:河沙(体积比2:2:1)。
In order to obtain a large number of propagules of Glomous mosseae (Gm), Gm was inoculated with plants of Sudangrass hyrid L. for 12 weeks. The propagation parameters of Gm The growth parameters of Sorghum sudanense, the physical and chemical properties of the substrate, the changes of the activity of urease, invertase and acid phosphatase. The results showed that the reproductive parameters and growth parameters of Gm were higher than those of other treatments. The contents of available nitrogen, available phosphorus, available potassium, pH and organic matter were 121.5 mg / kg, 13.47 mg / kg and 106.8 mg / kg, 6.9 and 32.3%. Correlation analysis showed that the colonization rate of Gm was positively correlated with mycorrhizal infection rate and spore density, negatively correlated with contents of available nitrogen, available potassium and organic matter before inoculation, positive correlation with available phosphorus and pH, The content of available nitrogen, available phosphorus, available potassium, organic matter, urease, sucrase and acid phosphatase activity of post-substrate was negatively correlated with pH. Infection rate and spore density were positively correlated with physical and chemical indexes before inoculation, and had the highest positive correlation coefficient with available phosphorus, followed by alkali-hydrolyzable nitrogen. Infection rate and spore density were positively correlated with alkali-hydrolyzable nitrogen, available potassium, pH, Organic matter and acid phosphatase activity was positively correlated with urease and invertase activity was negatively correlated. G.m fine reproductive matrix for the peat soil: field soil: river sand (volume ratio of 2: 2: 1).