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用不同浓度(0.05、0.10、0.20、0.50 mmol/L)的外源一氧化氮(nitric oxide,NO)供体硝普钠(sodiumnitroprusside,SNP)处理正常生长小麦(Triticum aestivum L.)叶片(二叶一心期时全展第一叶)。结果显示低浓度SNP(0.05、0.10、0.20 mmol/L)可以明显降低叶片H2O2和MDA的水平,其中0.10 mmol/L SNP的作用最为明显;而较高浓度SNP(0.50 mmol/L)则作用相反。进一步采用0.10 mmol/L SNP处理不同叶位的小麦叶片(四叶一心期),结果表明低浓度NO对不同老化阶段中叶片的H2O2、O2-.和MDA累积都有缓解作用,并明显减缓叶绿素、可溶性叶蛋白尤其是Rubisco的降解,有效延缓了叶片的老化进程。在完整叶绿体体外老化实验中也发现,不同浓度SNP(0.05、0.10、0.20、0.50、1.00、5.00 mmol/L)的作用同样表现双重性,其中0.20 mmol/L SNP对膜结构及Rubisco保护作用最明显。上述结果证实,低浓度外源NO可延缓小麦叶片的老化过程,并可能与其降低叶片活性氧(ROS)水平及缓解氧化损伤有关。
The leaves of normal wheat (Triticum aestivum L.) were treated with different concentrations of 0.05, 0.10, 0.20 and 0.50 mmol / L of sodiumnitroprusside (SNP) Ye Yi period when all the first leaf). The results showed that low concentrations of SNP (0.05,0.10,0.20 mmol / L) significantly reduced the level of H2O2 and MDA in leaves, of which 0.10 mmol / L SNP was the most obvious effect; while the higher concentration of SNP (0.50 mmol / L) . The results showed that low concentrations of NO could alleviate the accumulation of H2O2, O2- and MDA in leaves at different stages of aging and significantly slow down chlorophyll , Soluble leaf protein Rubisco degradation in particular, effectively delayed the leaf aging process. In vitro chloroplast aging experiments also found that different concentrations of SNP (0.05,0.10,0.20,0.50,1.00,5.00 mmol / L) also showed the duality of the role of which 0.20 mmol / L SNP on membrane structure and Rubisco protective effect obvious. The above results confirmed that low concentrations of exogenous NO can delay the aging process of wheat leaves, and may be related to its lower reactive oxygen species (ROS) levels and mitigation of oxidative damage.