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植物类受体蛋白激酶(receptor-likeproteinkinase,RLK)在高等植物生长发育和环境刺激的信号传导中起着重要的作用。本文报告了一个新的大豆类受体蛋白激酶基因的全长cDNA克隆及对其基因结构和功能的初步分析。研究表明该基因序列编码的蛋白包含一个跨膜域、一个具有丝氨酸/苏氨酸激酶活性的胞内域和一个缺少N-末端信号肽的胞外域。采用生物信息学方法分析表明,该基因与一些拟南芥菜类受体蛋白激酶基因具有很高的相似性,这些激酶N-末端都缺少信号序列,属于植物胞质类受体激酶(receptor-likecytoplasmickinase,RLCK)亚家族。因此命名该大豆基因为GmRLCK(GenBankAccessionNo.AY687390)。对GmRLCK激酶域中磷酸化可能性较高的位点进行了预测。RT-PCR的结果表明,GmRLCK在大豆子叶、根、花以及豆荚中都有较高的表达,而在胚根、茎和成熟叶片中的表达相对较弱。进化分析表明GmRLCK与一些衰老相关的植物类受体蛋白激酶具有较近的亲缘关系。
Receptor-like protein kinase (RLK) plays an important role in the signal transduction of higher plant growth and environmental stimuli. This article reports a novel full-length cDNA clone of the soy receptor protein kinase gene and preliminary analysis of its gene structure and function. Studies have shown that the protein encoded by this gene sequence contains a transmembrane domain, an intracellular domain with serine / threonine kinase activity and an extracellular domain lacking the N-terminal signal peptide. Bioinformatics analysis showed that this gene has high similarity with some protein kinase genes of Arabidopsis thaliana receptor. These kinases lack the signal sequence at the N-terminus and belong to the class of receptor-like cytoplasmic receptor kinase , RLCK) subfamily. Therefore, the soybean gene was named GmRLCK (GenBank Accession No. A687390). The site of phosphorylation in the GmRLCK kinase domain was predicted. The results of RT-PCR indicated that GmRLCK was highly expressed in soybean cotyledons, roots, flowers and pods, but relatively weak in radicle, stem and mature leaves. Phylogenetic analysis showed that GmRLCK has a close genetic relationship with some plant-related receptor protein kinases related to aging.