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目的 探讨半边旗不同部位、不同采集季节其抗肿瘤有效成分含量的变化。方法 用甲醇加热回流提取,反相高效液相色谱法定量分析。结果 一年中8、9、10、11四个月份采集有效成分11α-羟基-15-氧-16烯-对映贝壳杉烷-19-酸(5F)含量较高,不同部位有效成分的含量差异很大,叶片含量最高,是根茎的二十倍以上。结论采集季节最好选在8~11月份;根茎有效成分含量低,应采收地上部分,保留根茎,以利于资源保护。
Objective To investigate the changes of anti-tumor effective components in different parts of the semi-border flag and different collection seasons. Methods Heat reflux extraction with methanol, quantitative analysis by RP-HPLC. Results The content of active ingredient 11α-hydroxy-15-ox-16ene-enanthopereurane-19-acid (5F) was collected in the four months of the year 8, 9, 10, and 11 and the content of effective components in different parts was collected. The difference is very large, with the highest leaf content, which is more than twenty times that of rhizomes. Conclusion The collection season is best selected from August to November; the content of active ingredients of the rootstock is low, and the aerial part should be harvested to retain the rhizome to facilitate resource protection.