目的研究硝苯啶（Ｎｉｆ）、脑益嗪（Ｃｉｎ）、Ａ２３１８７和氯丙嗪（ＣＰＺ）对３Ｔ６成纤维细胞增殖的影响．方法以含１０％小牛血清的ＲＰＭＩ１６４０培养基培育３Ｔ６细胞，分别加入３种浓度的Ｃｉｎ，Ｎｉｆ，Ａ２３１８７和ＣＰＺ，处理２４ｈ后用图像分析仪和流式细胞仪检测３Ｔ６细胞的ＤＮＡ，ＲＮＡ和总蛋白以及细胞周期．结果Ｎｉｆ剂量依赖性地延迟３Ｔ６细胞Ｇ２期向Ｍ期的进展；Ｃｉｎ，Ａ２３１８７和ＣＰＺ分别呈剂量依赖性地抑制３Ｔ６细胞Ｇ１期向Ｓ期的进程；Ｎｉｆ，Ａ２３１８７和ＣＰＺ促进细胞的ＲＮＡ合成，Ｃｉｎ抑制细胞ＤＮＡ及ＲＮＡ的合成；Ａ２３１８７和ＣＰＺ（１０μｇ）抑制细胞蛋白质的合成，Ｃｉｎ（３０μｇ）及ＣＰＺ（５μｇ）促进细胞蛋白质合成．结论Ｎｉｆ等通过对细胞周期进程的延迟及引起细胞不平衡生长，抑制３Ｔ６成纤维细胞的增殖． Objective To investigate the effects of Nif, Cin, A23187 and CPZ on the proliferation of 3T6 fibroblasts. Methods 3T6 cells were cultured in RPMI1640 medium supplemented with 10% fetal bovine serum. Three concentrations of Cin, Nif, A23187 and CPZ were added respectively. After 24h treatment, the DNA and RNA of 3T6 cells were detected by image analyzer and flow cytometry Total protein and cell cycle. Results Nif delayed the G2-phase progression of 3T6 cells to M phase in a dose-dependent manner. Cin, A23187 and CPZ inhibited the progression of G1 phase to S phase in 3T6 cells in a dose-dependent manner. Nif, A23187 and CPZ promoted the cell RNA synthesis, Cin inhibited cell DNA and RNA synthesis; A23187 and CPZ (10μg) inhibited cell protein synthesis, Cin (30μg) and CPZ (5μg) to promote cellular protein synthesis. Conclusion Nif inhibits the proliferation of 3T6 fibroblasts by retarding the cell cycle progression and causing cell imbalanced growth.