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目的 研究LINC00263在内皮-单核细胞激活多肽-Ⅱ(endothelial monocyte activating polypeptide-Ⅱ,EMAP-Ⅱ)增加血肿瘤屏障通透性的作用及可能机制.方法 应用Real-time PCR检测人脑微血管内皮细胞(endothelial cells,ECs)和人胶质瘤微血管内皮细胞(glioma endothelial cells,GECs)中LINC00263的表达以及EMAPⅡ处理后LINC00263的表达.应用Lipo3000将LINC00263过表达和沉默质粒载体分别转染GECs,验证转染效率后,通过测量跨内皮细胞阻抗值(transendothelial electric resistance,TEER)和辣根过氧化物酶(horseradish peroxidase,HRP)渗透量,检测血肿瘤屏障通透性.应用Real-time PCR和Western blot检测紧密连接相关蛋白ZO-1(zonula occludens-1)、Occludin和Claudin-5的表达.结果 与ECs组相比,LINC00263在GECs中表达显著降低;EMAPⅡ作用后GECs中LINC00263的表达显著上调;与对照组相比,LINC00263(+)组TEER值显著降低,HRP渗透量显著升高,ZO-1、Occludin和Claudin-5的mRNA和蛋白表达水平显著降低;LINC00263(-)组的结果与之相反.结论 EMAPⅡ增强血肿瘤屏障的通透性可能与上调LINC00263的表达,降低ZO-1、Occludin和Claudin-5的表达相关.“,”Objective To investigate the effects and potential mechanism of LINC00263 in the process of endothelial monocyte-activating polypeptide-Ⅱ (EMAP-Ⅱ) increasing blood-tumor barrier(BTB) permeability.Methods The endogenous expression of LINC00263 was detected by Real-time PCR in endothelial cells(ECs) and glioma endothelial cells(GECs).GECs were treated with EMAP-Ⅱ for different times,Real-time PCR was used to detect the expression of LINC00263.LINC00263 overexpression and silencing plasmid vectors were transfected into GECs.After the transfection efficiency was tested,transendothelial electric resistance(TEER) and horseradish peroxidase(HRP) flux assays were used to test the permeability of BTB.The mRNA and protein expression levels of tight junction related proteins ZO-1 (zonula occludens-1),Occludin and Claudin-5 were evaluated by Real-time PCR and Western blot.Results The expression level of LINC00263 significantly decreased in GECs than in ECs,EMAP-Ⅱ up-regulated the expression level of LINC00263 in GECs.Compared with LINC00263(+)NC group,the value of TEER significantly decreased,HRP flux significantly increased and the expression levels of ZO-1,Occludin and Claudin-5 were obviously down-regulated in LINC00263(+) group,but quite opposite for LINC00263(-).Conclusion The increased permeability of BTB by EMAP-Ⅱ might be related to the upregulation of LINC00263 and downregulation of the expression levels of ZO-1,Occludin and Claudin-5.