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目的研究女性慢传输性便秘(slow transit constipation,STC)患者雌激素受体β(estrogen receptor β,ERβ)在乙状结肠组织的分布及其蛋白表达。方法采用免疫组织化学及Western blot方法检测20例STC患者以及20例对照组患者乙状结肠ERβ的分布及其蛋白表达。结果免疫组化结果显示ERβ在STC组及对照组乙状结肠黏膜、肌间神经丛及黏膜下神经丛均有表达,肌层未见表达。与对照组相比,STC组ERβ表达均明显降低,差异有统计学意义(P<0.05)。Western blot结果显示STC组乙状结肠ERβ蛋白表达较对照组明显降低,差异有统计学意义(P<0.01)。结论肌间及黏膜下神经丛ERβ蛋白表达降低可能参与STC发病。
Objective To study the distribution and expression of estrogen receptor β (ERβ) in the sigmoid colon of female patients with slow transit constipation (STC). Methods Immunohistochemistry and Western blot were used to detect the distribution and protein expression of sigmoid colon ERβ in 20 STC patients and 20 control subjects. Results Immunohistochemical results showed that ERβ was expressed in sigmoid colon mucosa, myenteric plexus and submucosal nerve plexus in STC group and control group, but not in myometrium. Compared with the control group, ERβ expression in STC group was significantly lower, the difference was statistically significant (P <0.05). Western blot results showed that the expression of ERβ in sigmoid colon in STC group was significantly lower than that in control group, with statistical significance (P <0.01). Conclusion The decrease of ERβ protein expression in intermuscular and submucosal plexus may be involved in the pathogenesis of STC.