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目的从北青龙衣醇提取物中分离胡桃苷B并建立了含量测定的方法。方法利用大孔树脂柱色谱、硅胶柱色谱和反相硅胶柱色谱进行分离纯化,得到胡桃苷B,通过高效液相色谱仪建立了含量测定的方法。结果经核磁共振谱鉴定胡桃苷B为(4S)-4,5-二羟基-α-四氢萘酮-4-O-β-D-吡喃葡糖苷,高效液相色谱洗脱条件为乙腈-水-磷酸(10∶90∶0.2)。结论该方法简便可行、重复性好,胡桃苷B可作为北青龙衣的指标成分建立质量控制的方法。
OBJECTIVE To separate and purify the content of Begonia B from the extract of Beilongqing alcohol. Methods The macroporous resin column chromatography, silica gel column chromatography and reversed-phase silica gel column chromatography were used to separate and purify the titanoside B, and the determination method was established by high performance liquid chromatography. Results The results of nuclear magnetic resonance spectroscopy identified as (4S) -4,5-dihydroxy-α-tetralone-4-O-β-D-glucopyranoside. The HPLC conditions were acetonitrile - water - phosphoric acid (10:90:0.2). Conclusion The method is simple and feasible with good reproducibility. Butroside B can be used as an index to establish the quality control method of Beilong Qingyi.