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在 SHIM-PACK CLC-ODS柱上 ,以甲醇与 0 .0 5 mol/L柠檬酸 + 0 .0 1mol/L 醋酸铵 (p H4.5 ) (V甲醇 ∶V柠檬酸 +醋酸铵=2 5∶ 75 )的混合溶液为流动相 ,采用反相高效液相色谱法分离测定了四种氟喹诺酮类药物 :环丙沙星、氧氟沙星、诺氟沙星、依诺沙星 ,线性范围分别为环丙沙星和氧氟沙星 10～ 10 0 μg/m L,诺氟沙星和依诺沙星 2～ 80μg/m L,相关系数 r>0 .9995 ,检出限分别为环丙沙星和依诺沙星 0 .42μg/m L,诺氟沙星 0 .36μg/m L ,氧氟沙星 0 .6 0μg/m L
On a SHIM-PACK CLC-ODS column, methanol and 0.05 mol / L citric acid + 0.01 mol / L ammonium acetate (pH 4.5) (V methanol: V citric acid + ammonium acetate = 25 : 75) as the mobile phase, the four fluoroquinolones were separated and determined by RP-HPLC: ciprofloxacin, ofloxacin, norfloxacin, enoxacin, linear range Respectively, 10 ~ 100 μg / m L of ciprofloxacin and ofloxacin, 2 ~ 80 μg / m L of norfloxacin and enoxacin, the correlation coefficient r> 0.9995, the detection limits were respectively ring Ciprofloxacin and enoxacin 0 .42μg / m L, norfloxacin 0.36μg / m L, ofloxacin 0. 6μg / m L