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目的研究锌指蛋白185(ZNF185)在小鼠睾丸精子、间质和支持细胞中的表达变化。方法应用免疫荧光组织化学染色法检测ZNF185在精子、间质、支持细胞和睾丸组织中的定位;实时定量PCR和Western blot法分别检测三种细胞中ZNF185 mRNA和蛋白表达水平的差异。结果免疫荧光细胞分析显示ZNF185在小鼠睾丸精子、间质和支持细胞中均表达,主要分布于间质和支持细胞胞质、精子细胞头部和尾部;实时定量PCR和Western blot结果显示,支持细胞ZNF185 mRNA和蛋白表达水平显著低于间质和精子细胞。结论 ZNF185分布于小鼠睾丸不同细胞,表达量存在差异。
Objective To study the expression of zinc finger protein 185 (ZNF185) in sperm, stroma and supporting cells of mice. Methods Immunofluorescence staining was used to detect the localization of ZNF185 in spermatozoa, mesenchyme, supporting cells and testicular tissues. Real-time quantitative PCR and Western blot were used to detect the difference of ZNF185 mRNA and protein expression. Results Immunofluorescence analysis showed that ZNF185 was expressed in the spermatozoa, stroma and supporting cells in testis, mainly distributed in the cytoplasm and the cytoplasm of the stromal cells and the sperm cells in the head and tail. Real-time PCR and Western blotting showed that ZNF185 Cell ZNF185 mRNA and protein expression levels were significantly lower than the interstitial and sperm cells. Conclusion ZNF185 is distributed in different testicular cells of mice, and the expression level is different.