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This study aims to produce an effective subunit vaccine against infectious bursal disease virus (IBDV).The genes ofchicken interleukin-2 (ChIL-2) and IBDV viral protein 2 (VP2) were amplified and fused by splice overlapextension-polymerase chain reaction (SOE-PCR).The fusion gene was digested by EcoR I/Kpn I and inserted intopBacPAK8 vector,resulting in recombinant transfer plasmid pBacPakVP2-IL2.The recombinant plasmid wastransfected into Sf-9 cells accompanied with hybrid nuclear polyhedrosis virus (HyNPV) genome DNA andlipofectin.Plaque-purification indicated that we had got the recombinant Hy-VP2-IL2.Fusion protein VP2-IL2was expressed effectively both in insect cells and bombyx mori.The expression of fusion protein was confirmed byELISA,SDS-PAGE and Western blotting assay,respectively.This efficient system allows us to meet the need forinexpensive vaccines required by the poultry industry.Cellular & Molecular Immunology.2005;2(3):231-235.
This study aims to produce an effective subunit vaccine against infectious bursal disease virus (IBDV). The genes ofchicken interleukin-2 (ChIL-2) and IBDV viral protein 2 (VP2) were amplified and fused by splice overlapextension-polymerase chain reaction -PCR). The fusion gene was digested by EcoR I / Kpn I and inserted in TopBacPAK8 vector, resulting in recombinant transfer plasmid pBacPakVP2-IL2. The recombinant plasmid wastransfected into Sf-9 cells accompanied with hybrid nuclear polyhedrosis virus (HyNPV) genome DNA andlipofectin . Plaque-purification indicated that we had got the recombinant Hy-VP2-IL2.Fusion protein VP2-IL2was expressed effectively both both in insect cells and bombyx mori. The expression of fusion protein was confirmed by ELISA, SDS-PAGE and Western blotting assays, respectively .This efficient system allows us to meet the need for inxpensive vaccines required by the poultry industry. Cellular & Molecular Immunology. 2005; 2 (3): 231-235.