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目的 :研究板蓝根高级不饱和脂肪组酸体外抗人肝癌BEL 74 0 2细胞的活性。方法 :MTT法测定细胞毒作用 ,集落形成实验观察药物对肝癌细胞增殖的抑制作用 ,PCR ELISA试剂盒测定细胞的端粒酶活性。结果 :板蓝根高级不饱和脂肪组酸能抑制BEL 74 0 2细胞的增殖 ,其IC50 为 0 6mg·mL-1,有促使肿瘤细胞向正常细胞逆转的趋势 ,可降低端粒酶活性的表达。结论 :板蓝根高级不饱和脂肪组酸具有体外抗肿瘤活性
Objective: To study the activity of Banlangen high unsaturated fatty acid against human hepatocellular carcinoma BEL 74 0 2 cells in vitro. Methods: MTT assay cytotoxicity, colony formation assay to observe the inhibitory effect of drugs on the proliferation of hepatocellular carcinoma cells, and the determination of telomerase activity by PCR ELISA kit. Results: The higher fatty acid of Banlangen could inhibit the proliferation of BEL-7402 cells with the IC50 of 0.6 mg · mL-1, which could reverse the tendency of tumor cells to normal cells and decrease the expression of telomerase activity. Conclusion: Banlangen high unsaturated fatty acid has anti-tumor activity in vitro