论文部分内容阅读
目的探讨粒细胞－巨噬细胞集落刺激因子（ＧＭ－ＣＳＦ）诱导小鼠红白血病细胞分化过程中，树突状细胞的产生及其抗原提呈功能的变化。方法采用流式细胞仪分析、电镜技术和４小时５１Ｃｒ释放法等，进一步观察了ＧＭ－ＣＳＦ的诱导分化作用。结果１００ｎｇ／ｍｌＧＭ－ＣＳＦ处理３天后的ＦＢＬ－３细胞，树突状细胞的特异性标志３３Ｄ１和ＮＬＤＣ１４５的表达阳性率显著升高；同时，ＭＨＣ－Ⅱ、Ｂ７－１、Ｂ７－２、Ｉ－ＣＡＭ－１、ＶＣＡＭ－１和ＣＤ４０表达水平均增加；扫描电镜显示ＧＭ－ＣＳＦ处理的ＦＢＬ－３细胞，表面出现许多树突状突起，透射电镜下可见细胞浆内有丰富的线粒体，核呈分叶状。同时，能够显著刺激同种异体Ｔ淋巴细胞增殖，促进ＩＬ－２的产生；可显著提高细胞毒性Ｔ淋巴细胞（ＣＴＬ）细胞对ＦＢＬ－３细胞的特异性杀伤活性。结论ＧＭ－ＣＳＦ诱导的红白血病细胞可向树突状细胞分化，本身具有抗原提呈功能。
Objective To investigate the changes of dendritic cells (DCs) production and antigen presenting function in erythroleukemia cell differentiation induced by granulocyte-macrophage colony-stimulating factor (GM-CSF). Methods Flow cytometry, electron microscopy, and 51-hour 4-hour release were used to further observe the differentiation of GM-CSF. Results The positive rate of dendritic cell specific markers 33D1 and NLDC145 was significantly increased in FBL-3 cells treated with 100 ng/ml GM-CSF for 3 days; at the same time, MHC-II, B7-1, B7-2, I- The expression of CAM-1, VCAM-1, and CD40 increased. Scanning electron microscopy showed that there were many dendritic processes on the surface of FBL-3 cells treated with GM-CSF, and there were abundant mitochondria in the cytoplasm under the transmission electron microscope. Leaflike. At the same time, it can significantly stimulate the proliferation of allogeneic T lymphocytes and promote the production of IL-2; it can significantly increase the specific killing activity of cytotoxic T lymphocyte (CTL) cells on FBL-3 cells. Conclusion The GM-CSF induced erythroleukemia cells can differentiate into dendritic cells and have antigen presentation function.