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目的:研究探讨玻璃化技术在根瘤菌保藏中的应用,并开展相关实验。方法:分别选取快生根瘤菌、慢生根瘤菌各1株为初始菌株,以30%甘油:15%乙二醇:15%DMSO为玻璃化冻存试剂,对大豆根瘤菌进行玻璃化冻存,分别选取冻存1个月、6个月以及12个月的菌株进行存活率以及固氮酶活性测定,研究玻璃化技术对根瘤菌生物学功能的影响。结果:保藏12个月后,大豆快生根瘤菌、慢生根瘤菌菌株存活率均可达到85%以上,固氮酶活性没有明显下降。结论:所用玻璃化方法及试剂对快生大豆根瘤菌保藏具有很好的作用,对慢生根瘤菌的保藏效果还需进一步实验验证。
Objective: To study the application of vitrification technology in the preservation of rhizobia and carry out related experiments. Methods: One strain of fast-growing rhizobia and one of slow-growing rhizobia were selected as the initial strain. Vitrification and cryopreservation were performed on soybean rhizobia with 30% glycerol, 15% ethylene glycol and 15% DMSO respectively Survival rate and nitrogenase activity of the strains that were frozen for 1 month, 6 months and 12 months were selected to study the effect of vitrification on the biological function of rhizobia. Results: After 12 months of storage, the viability of R. solanacearum and B. brasiliensis strains could reach more than 85%, and the nitrogenase activity did not decrease obviously. CONCLUSION: The vitrification methods and reagents used have good effect on the storage of fast-growing soybean rhizobia, and the further validation of the preservative effect on slow-growing rhizobia should be carried out.