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目的 :探讨溶血链球菌冻干制剂OK 4 3 2的抗肿瘤机理。方法 :B16黑色素瘤细胞接种于C5 7BL/ 6小鼠皮下 ,3d后腹腔注射 1KU的OK 4 3 2 ,每周 1次 ,连续 3周。观察肿瘤生长体积及荷瘤小鼠的生存期 ,并测定了OK 4 3 2在体内、体外对荷瘤小鼠脾细胞IL 2 ,IL 4 ,IL 6,IL 10 ,IL 12 ,IFN γ分泌的影响。结果 :OK 4 3 2能显著抑制B16黑色素瘤的生长 ,延长荷瘤小鼠的生存期 (P <0 .0 5 ) ;在体外OK 4 3 2可刺激荷瘤小鼠脾细胞IL 6,IL 10 ,IL 12 ,IFN γ的分泌 (P <0 .0 1) ;腹腔注射OK 4 3 2后荷瘤小鼠脾细胞IL 2 ,IL 12 ,IFN γ的分泌显著增加 ,而IL 10显著减少 (P <0 .0 5 ) ,提示OK 4 3 2治疗后荷瘤小鼠脾细胞Th1增加 ,Th2 相对减少。结论 :OK 4 3 2在体内可通过诱导荷瘤小鼠脾细胞IL 12的分泌 ,促进Th0向Th1分化 ,使宿主的免疫功能处于Th1优势状态 ,从而增强宿主的抗肿瘤作用
Objective : To investigate the antitumor mechanism of the freeze-dried formulation OK 4 32 of Streptococcus. Methods: B16 melanoma cells were inoculated subcutaneously in C57BL/6 mice. After 3 days, 1KU OK 4 3 2 was injected intraperitoneally once a week for 3 weeks. Observe tumor growth volume and survival time of tumor-bearing mice, and determine the secretion of IL2, IL4, IL6, IL10, IL12, and IFNγ from tumor-bearing mouse spleen cells by OK 4 32 in vitro and in vivo. influences. RESULTS: OK 4 32 significantly inhibited the growth of B16 melanoma and prolonged the survival time of tumor-bearing mice (P < 0.05). In vitro, OK 4 32 could stimulate the IL-6 of tumor-bearing mice. 10, IL 12, IFN γ secretion (P <0. 01); After intraperitoneal injection of OK 4 32, the secretion of IL 2, IL 12 and IFN γ in the spleen cells of tumor-bearing mice was significantly increased, while IL 10 was significantly decreased ( P <0.05, suggesting that the tumor cells in tumor-bearing mice had increased Th1 and decreased Th2 after OK 4 32 treatment. CONCLUSION : OK 432 can induce the secretion of IL 12 in tumor-bearing mouse spleen cells, promote the differentiation from Th0 to Th1, and make the host immune function in Th1 dominant state, thus enhancing the anti-tumor effect of the host.