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目的研发土壤中磷素的快速检测方法。方法针对土壤中磷含量的快速检测,利用比色法和光谱法构建了磷钼蓝反应的吸收曲线模型,并对一种智能土肥养份综合测试仪进行了测试。结果在检测波长为870nm时,将2.0 m L的26 g/L的钼酸铵溶液、1.0 m L的100 g/L抗坏血酸溶液和0.4 g/L的EDTA添加到酸性反应体系中,通过使用紫外-可见分光光度处理所测得的数据,可知磷溶液浓度与吸光度之间呈线性关系,得到线性回归模型为Y=0.01558X-0.1106,决定系数(r~2)=0.995。对这种智能土肥养份综合测试仪进行准确性测试,线性偏差在3%内,通过与所构建的线性回归模型的计算值进行对比,预测值误差在4%以内。结论所构建的线性回归模型是可用的,这种智能土肥养份综合测试仪可满足土壤中磷的快速检测要求。
Objective To develop a rapid method for the determination of phosphorus in soil. Methods Aiming at the rapid detection of phosphorus in soil, an absorption curve model of phosphomolybdenum blue reaction was established by colorimetry and spectrometry. A smart soil fertility comprehensive testing instrument was tested. Results 2.0 m L of 26 g / L ammonium molybdate solution, 1.0 m L of 100 g / L ascorbic acid solution and 0.4 g / L of EDTA were added to the acidic reaction system at the detection wavelength of 870 nm. By using UV - Visible spectrophotometric determination of the data obtained, the concentration of phosphorus solution and absorbance was linear between the linear regression model was Y = 0.01558X-0.1106, the determination coefficient (r ~ 2) = 0.995. The accuracy of this intelligent soil nutrient comprehensive tester was tested. The linear deviation was within 3%. Compared with the linear regression model, the error of prediction value was less than 4%. Conclusion The constructed linear regression model is available. This intelligent soil nutrient comprehensive tester can meet the requirement of rapid phosphorus detection in soil.