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按ＷＨＯ精液参数标准分析精液常规后，根据精子密度＞ ２０，１１ ～２０，１～１０（ ×１０６ 精子／ｍｌ） 和无精子将研究对象相应分为Ⅰ、Ⅱ、Ⅲ、Ⅳ组，每组２０ 例。用放射免疫法测精浆ＥＧＦ，并与有关参数进行相关分析。结果显示：Ⅰ、Ⅱ、Ⅲ、Ⅳ组精浆ＥＧＦ浓度分别是３ ．４４ ±１ ．０９ ，４．２２ ±１ ．１６，４．４１ ±１．７４ ，４ ．３３ ±１ ．４７（ｎｇ／ｍｌ） ，Ⅰ组显著低于Ⅱ、Ⅲ、Ⅳ组（Ｐ＜ ０．０５）；１ 次射精的ＥＧＦ水平分别是８．０１±３．３１，１０．４１±４．６１，９ ．７５ ±４ ．０７ ，９ ．６６±３ ．９５（ｎｇ／１次射精），组间比较没有显著差异；相关分析表明，精浆ＥＧＦ浓度（ｎｇ／ｍｌ） 与１ 次射精精子数呈显著负相关关系（ｒ＝ － ０．２５４８，ｎ＝ ６０ ，Ｐ＜０ ．０５），而在Ⅱ、Ⅲ组中，１ 次射精ＥＧＦ水平与精子计数呈显著正相关关系（ｒ＝ ０．３７１６ ，ｎ＝４０，Ｐ＜ ０．０２） ，精浆α糖苷酶、果糖、精子顶体酶无相关关系。
According to WHO semen parameters standard analysis of semen routine, according to the sperm density> 20, 11 ~ 20, 1 ~ 10 (× 106 sperm / ml) and azoospermia subjects were divided into Ⅰ, Ⅱ, Ⅲ, Ⅳ, 20 cases. The EGF of seminal plasma was measured by radioimmunoassay and related parameters were analyzed. The results showed that the concentrations of EGF in group Ⅰ, Ⅱ, Ⅲ and Ⅳ were 3. 44 ± 1. 09, 4.22 ± 1. 16,4.41 ± 1.74, 4. 33 ± 1. 47 (ng / ml), and the level of EGF in group Ⅰ was significantly lower than that in group Ⅱ, Ⅲ and Ⅳ (P <0.05). The level of EGF in group 1 was 8.01 ± 3.31 and 10.41 ± 4.61, 9. 75 ± 4. 07, 9. 66 ± 3. 95 ng / 1 ejaculation, there was no significant difference between the two groups. Correlation analysis showed that there was a significant negative correlation between the concentration of EGF in serum (ng / ml) and the number of sperm per sperm (r = - 0.2548, n = 60, P <0.05). In group Ⅱ and group Ⅲ, there was a significant positive correlation between EGF level and sperm count (r = 0.3716, n = 40, P <0.02) α glucosidase, fructose, sperm acrosin no correlation.